The anti lamin a c antibody reveals strong nuclear lamina staining while anti lamp1 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes.
Anti lamin immunofluorescence.
Lamin a c is cleaved by caspase 6 and serves as a marker for caspase 6 activation.
Tested in western blot wb immunofluorescence if immunoprecipitation ip and elisa elisa applications.
The antibody was developed using goat anti rabbit igg peroxidase secondary antibody and a chemiluminescent substrate.
Anti lamin a c terminal antibody is suitable for use in western blot 0 1 0 2 μg ml using hela nuclear extract.
By indirect immunofluorescence the antibody demonstrates specific basement membrane staining of enzymatically unmasked human and animal tissue.
The pictures were kindly supplied as part of the review submitted by dr josef gotzmann at medical university of vienna austria.
This antibody can also be used in indirect immunofluorescence 1 2 μg ml using hela cells rat nrk and mouse 3t3 cells.
Since both dna blue and lamin a c red are associated with the nuclear compartment this region appears crimson in this image.
The cells were methanol fixed 5 min and incubated with the antibody ab16048 1µg ml for 1h at room temperature.
2000 j struct biol 129 313 23.
Additionally anti lamin a c terminal antibodies are suitable for use in immunoblotting approx.
The cells were fixed with 4 paraformaldehyde for 10 minutes permeabilized with 0 1 triton x 100 for 10 minutes and blocked with 1 bsa for 1 hour at room temperature.
During apoptosis lamin a c is specifically cleaved into a large 41 50 kda and a small 28 kda fragment 3 4.
For immunofluorescence analysis hela cells were fixed and permeabilized for detection of endogenous lamin b1 using anti lamin b1 recombinant rabbit monoclonal antibody product 702972 1 100 dilution and labeled with goat anti rabbit igg h l superclonal secondary antibody alexa fluor 488 conjugate product a27034 1 2000.
Immunocytochemistry immunofluorescence anti lamin a antibody 133a2 ab8980 these images show hela cells stained with laminin a antibody ab8980 green and with dapi blue.
Invitrogen anti lamin b1 monoclonal l 5 catalog 33 2000.
This antibody reacts with chicken human mouse rat samples.
The cleavage of lamins results in nuclear dysregulation and cell death 5 6.
Antibody dilution 1 2 000 3.
Antibody dilution 1 1 000 2.
Supplied as 100 µg purified antibody 0 5 mg ml.
Specificity of the anti laminin antibodies is determined by indirect immunofluorescent labeling of formalin fixed paraffin embedded human or animal tissue sections and by dot blot immunoassay.
